However, Miron and colleagues [73] (2010) reported the frequency of PIK3CA activating mutations to be 30 % in pure DCIS (61 out of 202 cases) and mixed DCIS (29 out of 97 cases) and 29 % in invasive carcinoma (35 out of 120 cases). This evidence concerns the gene PIK3CA and ductal breast carcinoma in situ.