In order to assess whether miR-17 also regulates protein level of TRIM8 in glioma cells, we co-transfected U87MG cells with a pcDNA3-FLAG vector containing the ORF and 3′UTR of TRIM8 (hereafter named TRIM8-3′UTR), along with synthesized miR-17 or miR-17 inhibitor, respectively. Here, TRIM8 is linked to glioma.