Survival curves for the patients with ovarian cancer were stratified according to REG4 protein expression, based on the IHC results (Fig. 6d & e) and subsequent univariate analysis using Kaplan-Meier method indicated an inverse relationship between REG4 expression and cumulative (P = 0.038) or relapse-free survival rate (P = 0.033). This evidence concerns the gene REG4 and ovarian cancer.