Fig 2A) in the splenocytes significantly in both NCF1*/* and NCF1*/+ mice as compared to WT mice (Fig 2D). Interestingly, frequency of Treg increased significantly from peripheral blood to the spleen and from the blood to the tumor in both NCF1*/* and NCF1*/+ indicating the accumulation of Treg, (S2 Fig Bi, Bii) though no difference was found between NCF1*/* and NCF1*/+ mice in the tumor (Fig 2E). Here, NCF1 is linked to neoplasm.