In this regard, the mechanistic study of spinoculation-mediated HIV-1 infection revealed that centrifugation activates cofilin and subsequently triggers the dynamics of cortical actin in CD4 T cells, by which softens a major cytoskeletal barrier for HIV-1 entry and leads to a series of beneficial activities that enhance HIV-1 receptor mobilization and binding, virus entry, reverse transcription, and nuclear migration [14]. Here, CD4 is linked to HIV-1 infection.