Concentrations of ASA and SA as low as 100 μM significantly inhibited the formation of colonies in soft agar of high HMGB1-secreting MM cells (Figures 2a–d), whereas no effects were observed on the anchorage-independent growth of the low HMGB1-secreting MM cell line, PPM-Mill (Figures 2e and f). This evidence concerns the gene HMGB1 and Miyoshi myopathy.