Similarly, colocalization of CD4+ and CD8+ lymphocytes with F4/80+ macrophages increases over the course of infection (Fig 9A and 9C, S7 Fig middle and bottom rows), while the proportions of F4/80+ macrophages colocalizing with CD4+ or CD8+ lymphocytes decrease, as proportions of these cells decline (Fig 9B). Here, CD4 is linked to infection.