Similar to results in the IL-10-/- mouse model of chronic T cell-mediated colitis, E. faecalis virulence in organisms with only innate immune system was also mediated by both epaB and lgt. Injection of E. faecalis ΔepaB or Δlgt into Galleria (G.)mellonella resulted in an increased survival of larvae (Fig 3A), while both reconstituted mutants exerted virulence comparable to wild type E. faecalis (Fig 3B and 3C). The gene discussed is IL10; the disease is colitis.