Similarly, markers for other immune cells, such as T cells (Cd28, Csf2, Cd4, Ccr5, Gata3 Cxcr4), B cells (Pax5, Cd70, Cd79b), and leukocytes (Cd33, Cd52, Cd53, Cd44, Prg2) were also not increased (data not shown), suggesting the absence of significant immune cell infiltration and steatohepatitis in these mice after 8 weeks on the HF/HS diet. Here, CD53 is linked to hydrops fetalis.