To analyse the indels generated by somatic gene transfer of gPtch1/Cas9 in greater detail, we performed targeted ultra-deep sequencing of the Ptch1 locus in three different tumours (two CRISPR-Ptch1 (EP), one CRISPR-Ptch1 (TF); Supplementary Table 1) and matched normal tissue using five different staggered amplicons (251 bp paired-end, on average >200,000-fold coverage per amplicon; Supplementary Data 1). Here, PTCH1 is linked to neoplasm.