The mRNA levels of GYS1 (the main GYS isoform expressed in the testicles) were significantly decreased by 20% in the testicles of T2DM rats (0.80 ± 0.01-fold variation) when compared to rats of the control group, while its protein levels were not significantly altered (Figures 4(a) and 4(b)). This evidence concerns the gene GYS1 and type 2 diabetes mellitus.