The average Dppa4 expression (ΔCt value) in the colon tumor group was 5.17 ± 0.37 whereas in the normal tissue group, it was 8.90 ± 0.55 (P < 0.01), indicating that the Dppa4 mRNA level was upregulated in cancer tissues than in paired normal mucosa. This evidence concerns the gene DPPA4 and colonic neoplasm.