In univariate analysis, patients with Dppa4-positively stained colon tumors had an obviously lower DFS and OS than those with Dppa4-negative tumors [DFS, HR 6.118 (95 % CI 3.004–12.462); OS, HR 6.348 (95 % CI 2.875–14.014)] (Table 2). This evidence concerns the gene DPPA4 and colonic neoplasm.