In the present study, we provided evidence that miR-29b expression in high-metastatic CD133-positive A549 lines was down-regulated when compared to miR-29b expression in paired low-metastatic CD133-negtive A549 cell lines, miR-29b was confirmed directly targeted 3’-UTR of PTEN and MMP2 mRNAs and down-regulated MMP2 protein expression to suppress lung cancer metastasis in vitro and in vivo. Here, PROM1 is linked to lung cancer.