Notably, we observed even more significant differences in aged, 20-month-old mice between the WT and fat-1 groups in the markers of metabolic endotoxemia, inflammation, and metabolic syndrome, as well as downstream factors of the pathways of LPS-TLR4 (LBP, CD14, TLR4 and NFκB-p65) and LPS-NLRP3 inflammasome (NLRP3, ASC, Caspase-1 and Pannexin) (Supplementary Fig. S2). This evidence concerns the gene TLR4 and metabolic syndrome.