However, following the second skin infection with S. aureus isolate P, the IgG levels against IsaA, Nuc, PrsA, and WTA were significantly elevated at week 5 (P < 0.002), whereas the second skin infection with S. aureus 8325–4 resulted in a significant rise in IgG levels at week 5 against Efb, IsaA, and IsdA (P < 0.002). The gene discussed is NUCB1; the disease is skin infection.