The aim of the present study was to establish the optimal timing, between the beginning of FSH administration and the beginning of GnRH-ant administration, and the optimal dose (75 IU, vs. 150 IU) of rLH administration in EPR infertile females undergoing IVF cycle using GnRH-ant, in order to achieve the greatest number of retrieved oocytes, optimal oocyte maturation degree and fertilization rate, optimal embryo quality, optimal endometrial thickness at embryo-transfer and the highest pregnancy rate. The gene discussed is GNRH1; the disease is Infertility.