We conducted a multicenter study in 13 medical centers in Taiwan to assess methylation biomarkers (SOX1, PAX1, ZNF582, PTPRR, AJAP1, HS3ST2, POU4F3, and ADRA1D genes) to detect CIN3+/CIS and complex hyperplasia of the endometrium in AGC identified on Pap smear for invasive procedures. Here, SOX1 is linked to in situ carcinoma.