In this study, we showed for the first time that HIF-1α expression directly regulated ZEB1, and positively correlated with the expression of ZEB1 and Vimentin, and inversely correlated with E-cadherin in primary and metastatic CRC tissue samples, which suggest co-expression of HIF-1α, ZEB1, Vimentin and decreased of E-cadherin may be used as a valuable marker to predict the metastastic potential of CRC patients. This evidence concerns the gene ZEB1 and colorectal carcinoma.