In this study, we use MUC1 gene-silenced and overexpressing HCC cells to determine if MUC1 can promote the migration and invasion of HCC cells and clarify whether TGF-β/Smad2 and JNK are involved in the mechanisms that MUC1 promotes the migration and invasion of HCC cells, providing a novel therapeutic target for the pathogenesis and gene therapy of HCC. The gene discussed is MUC1; the disease is hepatocellular carcinoma.