MUC1 and hepatocellular carcinoma: To determine the effect of MUC1 on the migration and invasion of HCC cells, wound-healing, transwell migration, and matrigel invasion assays were performed using two independent MUC1-knockdown clones (MR1-D4 and MR1-D9) and two MUC1-overexpressing cell lines (Bel-7402-MUC1 and Hep3B-MUC1) that were previously established [22, 23].