Using the COLO-205 as the cell model for further studies, our data suggest that FA inhibited proliferation of COLO-205 through activating the c-SRC/ERK1/2/NFκB/TP53 signaling pathway mediated by FRα and FA nutritional supplementation inhibited COLO-205 colon tumor growth and prolonged the life span of tumor bearing mice. The gene discussed is MAPK3; the disease is colonic neoplasm.