In line with the negative effect of intracellular CE content on vascular cell migration, we could not detect a relation of intratumoral CE accumulation with migration and invasion markers (MMP9, MMP2, TIMP and CTSS) in breast tumors, nor with clinicopathological characteristics of invasion (TNM stage, number of lymph nodes affected, and vascular invasion). This evidence concerns the gene CTSS and breast neoplasm.