In general, the mutation rates obtained by our NGS assay for genes known to be mutated in CLL (TP53, SF3B1, ATM, NOTCH1, XPO1, MYD88, DDX3X) are comparable to other studies [6–8, 10, 13, 17–19]. This evidence concerns the gene MYD88 and B-cell chronic lymphocytic leukemia.