MYCN expression was undetectable in GI-ME-Nkd-3650, GI-ME-Nkd-1463 and GI-ME-Nkd-LacZ cells by Western Blotting and real-time quantitative PCR (Figure 11E), thus further corroborating the conclusion that the tumor promoting effect of ATM silencing in the NB cell background does not require MYCN. This evidence concerns the gene ATM and neoplasm.