Both CD4+ T helper1 (Th1) cells and CD8+ CTLs are required to control HCMV replication during reactivation after HSCT.20–22 A 16-hour IFN-γ catch assay based on flow cytometry analysis of cryopreserved/thawed selected CD3+ T cells was used to evaluate whether SmyleDCpp65 (harvested on day 7 after transduction) could activate both types of T cells obtained from HCMV seropositive HD (n = 3) (Figure 3a). The gene discussed is IFNG; the disease is Huntington disease.