To examine the contribution of e2f3, e2f8 and fabp3 to the development of hepatic steatosis, we conducted fabp3, e2f3 and e2f8-specific MO (MO-fabp3, MO-e2f3 and MO-e2f8) i.p. administration every week during feeding experiments of DIO-zebrafish as previously described [26]. Here, E2F3 is linked to fatty liver disease.