However, with anti-obese compounds, AC suppressed hepatic steatosis, consistent with mammals, through suppression of e2f3, e2f8 and fabp3. Additionally, anserine and creatine administration to DIO-zebrafish ameliorated the hepatic steatosis with down regulation of e2f8 and fabp3 in the current study. The gene discussed is E2F8; the disease is fatty liver disease.