To the best of our knowledge, the present report is the first to demonstrate the modulatory effects of differentiation on factors that have an important role in physiopathology of CLL, including LEF1, TCL1, ROR1, FMOD, TNFRSF13B, PI3K, BTK, p27, BCL2, BCLXL, PUMA and MCL1. This evidence concerns the gene TNFRSF13B and B-cell chronic lymphocytic leukemia.