Because p53 activates CDKN1A gene transcription and its expression reflects the functional status of p53 protein [25, 26], the CDKN1A expression was measured and compared with that of RAD54B. This analysis was limited to samples without any p53 hotspot mutations observed in CRC because most of such p53 mutants lack the normal transcriptional activities [27, 28]. Here, TP53 is linked to colorectal carcinoma.