Therefore, we screened TF, proto-oncogenes, tumor suppressor genes, and other TAG from the genes adjacent to aberrant DNA methylation sites, of which multiple known cancer related genes, including MYC, DDR1, MEF2C, NDRG2, SIX1, TNFRSF10B and TSGA10, had HyperM phenomena. The gene discussed is SIX1; the disease is cancer.