Based on the overall metabolite profiles, control and MUC16 knockdown cultured cells, as well as MUC16-high and MUC16-low human pancreatic tumors, segregated in distinct clusters, in a MUC16 expression-dependent manner, in the respective 2D-PLS-DA (partial least squares discriminant analysis) plots (Figure 6A and 6D). This evidence concerns the gene MUC16 and pancreatic neoplasm.