In conclusion, we have demonstrated that 1) FFPE biopsy samples of HCC can be used for targeted DNA and RNA sequencing, 2) the tumor mutational burden is a candidate predictor of sorafenib effectiveness, and 3) the tumor gene expressions of NRG1, TGFa, and PECAM1 are candidate markers for the prediction of sorafenib effectiveness. The gene discussed is NRG1; the disease is hepatocellular carcinoma.