7 Additionally, a gain-of-function mutant of TRPV4 has been shown to augment Ca2+ entry and decrease cell viability in transfected HEK293 cells.8 TRPV4 can be activated by cell swelling-induced mechanical stimulation and metabolites of AA that are always associated with cerebral ischemia. The gene discussed is TRPV4; the disease is brain ischemia.