Totally 28 miRNAs altered by rh-endostatin and 42 miRNAs altered by gemcitabine respectively were determined by high-throughput sequencing analysis and miR-19a was down-regulated significantly in both groups, confirmed also by fluorescence quantitative RT-PCR in ASPC-1 cells (Figure 1C) and the other two pancreatic cancer cell lines (Panc-1 and Capan-2 cells, Figure 1D). This evidence concerns the gene COL18A1 and familial pancreatic carcinoma.