Western blot analysis of protein extracts from the laser-dissected photoreceptors of P28 retina revealed that infection with AAV-mCAR-EGFP-triple sponge resulted in a marked increase in Ddx3x protein level compared with retina infected with the AAV-mCAR-EGFP/control, indicating that Ddx3x is also regulated by miR-183/96/182 in vivo (Supplementary Fig. 3e). This evidence concerns the gene DDX3X and infection.