The peaks obtained by ChIP-seq and used for the motif alignment were confirmed by ChIP-qPCR with anti-SOX9 and anti-NF-YA antibodies in separate ChIP analysis of HT29 cells (Supplementary Figure S5A and S5B) and HCT116 cells (data not shown), which validated the ChIP-Seq data for regions highly enriched for SOX9 and NF-YA binding and confirmed that the findings were consistent in two colorectal cancer cell lines. This evidence concerns the gene SOX9 and colorectal cancer.