The out-of-frame clone E13519 with a sequence homology to oxidase (cytochrome c) assembly 1-like (OXA1L) showed the highest AUC value (AUC = 0.782) specific for the discrimination of BPH and normal sera, i.e., higher immunogenicity in normal sera than in BPH sera. This evidence concerns the gene OXA1L and benign prostatic hyperplasia.