For the discrimination of BPH and normal sera the clone E19574 with sequence homology to the pseudogene similar to ubiquitin and ribosomal protein S27a precursor was identified as the most informative in-frame clone with AUC < 0.3, i.e., higher immune response in sera of BPH patients than in normal sera (AUC = 0.175). This evidence concerns the gene RPS27A and benign prostatic hyperplasia.