The qRT-PCR experiments were designed to detect the expression levels of the top 5 ranking HAX1, RNF2, PIM3, TPP1 and CAV1 genes in addition to the seed proteins ABCB1, AKT1 and BCL2 in both the SGC7901 gastric cancer cell line and the Adriamycin resistant SGC7901/ADR cell line (Fig. 3a). Here, AKT1 is linked to gastric cancer.