Inhibition of 12-LOX activity leads to apoptosis in Walker 256 carcinosarcoma cells, whereas overexpression of 12-LOX in the same cell type results in up-regulation of the anti-apoptotic protein Bcl-2 [28], while in MCF-7 breast cancer cells, overexpression leads to increased cellular proliferation in nude mice [35]. This evidence concerns the gene ALOX12 and breast cancer.