Quantitative real-time PCR of SEMA3E in a panel of 26 pairs of tissues agreed with the initial PCR result, and obvious down-regulation of SEMA3E mRNA was observed in 21/26 (80.77%) gastric cancer tissues when the cut-off was set as 1(Fig. 1B and 1C, P < 0.001). The gene discussed is SEMA3E; the disease is gastric cancer.