NR4A1 and malignant colon neoplasm: ACHN cells were transfected with an NBRE-luc construct containing 3 monomer binding sites and both DIM-C-pPhOH and DIM-C-pPhCO2Me significantly decreased luciferase activity (Fig 1D) as previously described in colon cancer cells [17], demonstrating NR4A1 antagonist activity in this transactivation assay.