In order to differentiate ALS-iPSCs into spinal cord populations containing MNs, we adapted a previously established differentiation protocol that includes an initial phase of neural induction followed by the regional specification by retinoic acid (RA) and sonic hedgehog (SHH) (Wichterle et al., 2002; Hu and Zhang, 2010). This evidence concerns the gene SHH and amyotrophic lateral sclerosis.