Within these datasets, a ‘developmental cascade’ can be recognized; terms uniquely enriched in the Nes-Cre Wt1co/co samples are related to muscle cell proliferation and differentiation, in the overlap between the Nes-Cre Wt1co/co and WT1-mutant tumours for filament assembly and uniquely in the WT1-mutant tumours for muscle-function-related terms, such as contraction and relaxation, as well as differentiation into specific muscle types. This evidence concerns the gene NES and neoplasm.