To further examine whether EGF treatment was sufficient to upregulate Tensin4 expression in HCC cells with relatively low Tensin4 expression, PLC (very low expression) and BEL7402 (low expression) HCC cells were subjected to prolonged EGF treatment for 24 hours, with SMMC-7721 cells serving as a control. This evidence concerns the gene EGF and hepatocellular carcinoma.