MGMT and glioblastoma: The miRs identified by the first screening were validated by in silico analysis of putative binding sites in the 3′-UTR region of MGMT gene utilizing different algorithms, which restricted the potentially relevant miRs to a limited series, that was verified for inverse correlation of expression with MGMT in the Chinese Cancer Genome Atlas, then tested in LN340 glioblastoma cell line in vitro (122).