Since a decreased expression of GL1, a member of the ATG8 family, has been frequently observed in breast cancer (BC) cells, we decided to use the MCF-7 cell line, which has been described to present an undetectable expression of GL1 in western blotting and a weak basal signal in IF, to validate the specificity of the SQSTM1/GL1 and NIX/GL1 P-LISA (Fig 3). This evidence concerns the gene BNIP3L and breast carcinoma.