To further confirm whether NF-κB activation induced by PinX1 inhibition caused the up-regulation of MMP-2 in human ccRCC cells, Western blot analysis showed that p65 siRNA can inhibited the expression of p65 and MMP-2 in ccRCC cells (Figure 4g and 4h). This evidence concerns the gene PINX1 and nonpapillary renal cell carcinoma.