The migration and invasion ability can be enhanced by inhibiting of PinX1 in ccRCC cells, however, these regulations were blocked by MMP-2 selective inhibitor I. Above all, it was confirmed that PinX1 inhibited ccRCC cells’ migration and invasion by suppressing MMP-2 expression and activity (Figure 3f and 3g). This evidence concerns the gene MMP2 and nonpapillary renal cell carcinoma.