For this purpose, we analyzed Sema3C mRNA levels by using quantitative real-time PCR (RT-PCR) technique on mRNA isolated from the same set of glioma samples tha has been used for Western blot studies except for 10 samples, which were not included because of the scarcity of the material or due to the exceed value of standard deviation of CT. The gene discussed is SEMA3C; the disease is glioma.