In vivo, the activation of TLR4 and TLR4-downstream molecules (TRAF6, NF-κB and AP-1) in spleens of B10 (TLR4+/+) and ScCr (TLR4−/−) tumor-bearing mice were measured by Q-PCR and Western blot. The gene discussed is NFKB1; the disease is neoplasm.