Twenty five days after administration, TLR4, TRAF6, NF-κB and AP-1 in PSP and LPS group (positive control) were significantly increased at mRNA and protein level relative to those of the saline group in the spleen of B10 (TLR4+/+) tumor-bearing mice (all p < 0.05). The gene discussed is TLR4; the disease is neoplasm.