This study was conducted on a randomly selected group of women with unexplained infertility seeking ICSI/IVF, aiming to detect the prevalence of Chlamydial infection using ELISA for detection of anti-C.trachomatis IgG and IgM and Real-TM PCR for Chlamydial antigen detection. This evidence concerns the gene CD40LG and chlamydia trachomatis infectious disease.