In this study, we first reported upregulation of Hif-1α by administration of anti miR-335 in the late time of cerebral ischemia and displayed that induction of anti miR-335 at 24 hrs post-occlusion could upregulate Hif-1α expression and bring about reduction of infarct volume (Fig 4D). The gene discussed is HIF1A; the disease is brain ischemia.