To identify potential local differences of E-cadherin (CDH1) expression in liver sections of HCC patients, we prepared ISH probes for the detection of CDH1 mRNA, following our rapid protocol for the preparation of antisense RNA probes.14 These probes were subsequently used to visualize CDH1 mRNA distribution in complementary liver sections from HCC patients representing CAs and NCAs. This evidence concerns the gene CDH1 and hepatocellular carcinoma.