It is unclear how downregulation of E-cadherin occurs at the level of individual cells within the tumor tissue; therefore, we decided to analyze the expression of E-cadherin (CDH1) in hepatocellular carcinomas by in situ hybridization (ISH) and fluorescent immunohistochemistry (FIHC) of liver sections from patients, using matched tissue sections for cancerous (CA) and noncancerous (NCA) tissue areas. Here, CDH1 is linked to hepatocellular carcinoma.